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KMID : 0368419930360030241
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Journal of Plant Biology
1993 Volume.36 No. 3 p.241 ~ p.249
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Changes in RNA Synthesis During Male Gametogenesis of Brassica napus
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Abstract
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The pattern of RNA synthesis during male gametogenesis of Brassica napus was studied using ^3H-uridine autoradiography. No incorporation of isotope occurred in the newly released microspore and the nonvacuolate, furrowed, microspore. Peak incorporation of label during male gametogenesis occurred in the uninucleate microspores showing various degrees of vacuolation. In this microspore stage, silver grains were localized in the nucleus and cytoplasm. Moderated incorporation of the isotope occurred in the nucleus of the vacuolated microspore. After the micropore mitosis, isotope incorporation ocurred predominantly in the nucleus of the vegatative cell with little or no incorporation in the generative cell. In tricellular pollen, no incorporation of isotope was observed in both the vegetative nucleus and the sperms. Silver grains almost completely disappeared from tricellular mature pollen grains ready to germinate.
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